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KMID : 0379119880160040253
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Korean Journal of Mycology
1988 Volume.16 No. 4 p.253 ~ p.254
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Dynamics and Ultrastructure of Nuclear Mobility in Pleurotus ostreatus (Basidiomycetes)
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Yoon Kwon-Sang
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Abstract
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Nuclear migration is a widespread phenomeon of considerable developmental significance in diverse life cycle stages of virtually all fungi and, indeed, most eukaryotes. Ultrastructural, inhibitor and genetic data suggest that microtubules are involved in generating the forces needed for nuclear migration but the mechanisms by which they work are totally obscure. One approach to elucidating such mechanisms is by a detailed ultrastructural analysis of the microtubules associated with migrating nuclei, but to be valid such an analysis requires fixations which faithfully preserve all microtubules in an undisturbed configuration and a high degree of certainty about the precise behaviour of the nucleus at the time of fixation. Freeze-substitution is the best currently available fixation protocol whose use for much work is mandatory but it cannot be employed on nuclei undergoing observation at the time of fixation.
Consequently we have investigated and characterized a highly predictable nuclear motility system wherein the prior in viva behaviour of fixed nuclei can be infered with a high degree of confidence. This talk presents the results to data of a light and electron microscopy investigation of living and freezesubstituted nuclei of Pleurotus ostreatus undergoing post-mitotic migration from the clamp connections.
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